O2 and H2O are each the source of one O in NO− 2 produced from NH3 by Nitrosomonas: 15N‐NMR evidence

FEBS Letters

Hooper, Alan B.¹  Andersson, Kristoffer K.¹ 

[1] Department of Genetics and Cell Biology, University of Minnesota, St. Paul, MN 55108, USA

关键词: Nitrosomonas;    15N-NMR;    Nitrite;    Ammonia;    18O Isotope shift;    Nitrification;    CCCP;    carbonyl cyanide m-chlorophenylhydrazone;    PMS;    N-methylphenazonium methosulfate;   

DOI  :  10.1016/0014-5793(83)80292-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The exchange of ¹⁸O between H₂ ¹⁸O and exogeneously added ¹⁵N¹⁶O⁻ ₂ which occurs during oxidation of ammonia by Nitrosomonas is shown to occur one oxygen at a time. Conditions in which the exchange is diminished (notably the presence of ¹⁴NO^– ₂ and CCCP) allowed demonstration that water and dioxygen are each the source of one oxygen in nitrite produced from ¹⁵NH₃. The nitrate produced in the presence of ¹⁸O₂ consisted of 67 and 0% ¹⁵N¹⁸O¹⁶O⁻ and ¹⁵N¹⁸O¹⁸O⁻, respectively. Analysis was made using the ¹⁸O-isotope shift in ¹⁵N-NMR.

【 授权许可】

Unknown