Solution conformation of biantennary complex type oligosaccharides

FEBS Letters

Fernandes, D.L.¹  Rademacher, T.W.¹  Dwek, R.A.¹  Homans, S.W.¹ 

[1] Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, England

关键词: Oligosaccharide conformation;    Nuclear Overhauser effect;    Human serum transferin Two-dimensional NMR spectroscopy;   

DOI  :  10.1016/0014-5793(83)80291-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using data obtained from both one-dimensional and two-dimensional Nuclear Overhauser effect measurements, we have extended our original observations [FEBS Lett. (1983) 150, 503–506] upon the preferred conformer of the complex-type oligosaccharide unit derived from human serum transferrin. We propose that the Galβ1—4GlcNAcβ1—2Manα1—3Manβ1—4GlcNAcβ1—4GlcNAc unit has continuous secondary structure, and that the α(1–6) antenna extends from this structure with no single preferred orientation. Within the α(1–6) antenna, however, a segment of secondary structure (Galβ1–4GlcNAcβ1–Manα1–) exists with a preferred conformation which is probably identical to that found in the α(1–3) antenna.

【 授权许可】

Unknown