Solution conformation of the biantennary N‐linked oligosaccharide of human serotransferrin using 1H NMR nuclear Overhauser effect measurements

FEBS Letters

Fernandes, D.L.¹  Rademacher, T.W.¹  Dwek, R.A.¹  Homans, S.W.¹ 

[1] Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, England

关键词: Oligosaccharide;    Oligosaccharide conformation;    Nuclear Overhauser effect;    Nuclear magnetic resonance;    Human serotransferrin;    NOE;    nuclear Overhauser effect;    NMR;    nuclear magnetic resonance;    HST;    human serotransferrin;    Man;    D-mannopyranose;    Gal;    D-galactopyranose;    GlcNAc;    2-acetamido;    2-deoxy D-glucopyranose;   

DOI  :  10.1016/0014-5793(82)80799-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The conformation in solution of the biantennary complex type oligosaccharide unit derived from human serotransferrin has been investigated using ¹H—¹H Nuclear Overhauser Effect (NOE) measurements at 300 MHz. From quantitation of the NOE, the α(1–3) antenna is shown to exist in a preferred solution conformation with respect to the mannosyl-chitobiose core. The flexibility of the α(1–6) arm, together with the absence of NOE data between this arm and the core, indicates that, in contrast to the α(1–3) arm the α(1–6) arm has no preferred conformation with respect to the core.

【 授权许可】

Unknown