The lactose carrier of Escherichia coli functionally incorporated in Rhodopseudomonas sphaeroides obeys the regulatory conditions of the phototrophic bacterium

FEBS Letters

Konings, Wil N.¹  Elferink, Marieke G.L.¹  Hellingwerf, Klaas J.¹  Kaplan, Samuel²  Nano, Francis E.² 

[1] Department of Microbiology, University of Groningen, Biological Centre, Kerklaan 30, 9751 NN Haren, The Netherlands;Department of Microbiology, University of Illinois, Urbana, IL 61801, USA

关键词: Allosteric regulation;    Conjugation;    Escherichia coli;    Lactose carrier;    Rhodopseudomonas sphaeroides;    Solute transport;   

DOI  :  10.1016/0014-5793(83)80046-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Rhodopseudomonas sphaeroides was provided with the ability to transport lactose via conjugation with a strain of Escherichia coli bearing a plasmid containing the lactose operon (including the lac Y gene, coding for the lactose carrier or M protein) and subsequent expression of the lac operon in Rps. sphaeroides (Nano, F.E. and Kaplan, S. submitted). The initial rate of lactose transport in Rps. sphaeroides was studied as a function of the light intensity and the magnitude of the proton-motive force. The results demonstrate that lactose transport is regulated by the rate of cyclic electron transfer in the same way as the endogenous transport systems.

【 授权许可】

Unknown