Does the lactose carrier of Escherichia coli function as a monomer?

FEBS Letters

Weigel, Ulrich¹  Müller-Hill, Benno²  Lustig, Ariel³  Keith Wright, J.¹  Bocklage, Hubertus²  Mieschendahl, Martin²  Overath, Peter¹ 

[1] Max-Planck-Institut für Biologie, Corrensstr. 38, D-74 Tübingen, FRG;Institut für Genetik der Universität zu Köln, Weyertal 121, D-5000 Köln 41, FRG;Abteilung für biophysikalische Chemie, Biozentrum, Universität Basel, CH-4056 Basel, Switzerland

关键词: Lactose carrier;    M r-value;    Detergent micelle;    lacY −d mutant;    Negative dominance;    DodOMalt;    dodecyl-O-β-D-maltoside;    NpβGal;    o-nitrophenyl-β-D-galactopyranoside;    GalSGal;    β-D-galactosyl-1-thio-β-D-galactopyranoside;   

DOI  :  10.1016/0014-5793(83)81039-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The purified lactose carrier of Escherichia coli (product of the lacY gene) is shown to be a monomer in detergent micelles of dodecyl-O-β-D-maltoside. The negative-dominant phenotype of mutant carriers (lacY ^−d mutants) could not be verified by measurements of the rate of galactoside transport in lacY ⁺/Y ^−d diploid strains. It is proposed that the membrane-embedded carrier functions as a monomer in galactoside-H⁺ symport.

【 授权许可】

Unknown