Binding of actinomycin D to DNA revealed by DNase I footprinting

FEBS Letters

Beabealashvilli, R.Sh.¹  Scamrov, A.V.¹ 

[1] USSR Cardiology Research Center, USSR Academy of Medical Sciences, 3 Cherepkovskaya Str., 15 A, Moscow 121522, USSR

关键词: Actinomycin D;    Promoter lac UV5;    DNA-ligand interaction;    Footprint;    DNase I;   

DOI  :  10.1016/0014-5793(83)80027-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have analyzed the specificity of the actinomycin D—DNA interaction. The ‘footprint’ method has been used in this investigation. It is shown that: (i) The presence of dinucleotide GC or GG is required for binding of a single drug molecule. (ii) The strong binding sites are encoded by tetranucleotide XGCY; where X ≠ G and Y ≠ C in accordance with RNA elongation hindrance sites [1]. (iii) There is a positive cooperativity in binding of actinomycin D with DNA.

【 授权许可】

Unknown