期刊论文详细信息
FEBS Letters
Binding of actinomycin D to DNA revealed by DNase I footprinting
Beabealashvilli, R.Sh.1  Scamrov, A.V.1 
[1] USSR Cardiology Research Center, USSR Academy of Medical Sciences, 3 Cherepkovskaya Str., 15 A, Moscow 121522, USSR
关键词: Actinomycin D;    Promoter lac UV5;    DNA-ligand interaction;    Footprint;    DNase I;   
DOI  :  10.1016/0014-5793(83)80027-1
学科分类:生物化学/生物物理
来源: John Wiley & Sons Ltd.
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【 摘 要 】

We have analyzed the specificity of the actinomycin D—DNA interaction. The ‘footprint’ method has been used in this investigation. It is shown that: (i) The presence of dinucleotide GC or GG is required for binding of a single drug molecule. (ii) The strong binding sites are encoded by tetranucleotide XGCY; where X ≠ G and Y ≠ C in accordance with RNA elongation hindrance sites [1]. (iii) There is a positive cooperativity in binding of actinomycin D with DNA.

【 授权许可】

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