The activation‐dependent induction of APN‐(CD13) in T‐cells is controlled at different levels of gene expression

FEBS Letters

Ansorge, S¹  Arndt, M¹  Lendeckel, U¹  Wex, Th¹  Bühling, F¹  Frank, K¹ 

[1] Institute of Experimental Internal Medicine, Department of Internal Medicine, University of Magdeburg, Leipziger Str. 44, D-39120 Magdeburg, Germany

关键词: CD13;    E.C. 3.4.11.2;    Actinomycin D;    Half-life time;    APN;    aminopeptidase N;    Ala-pNA;    alanine-p-nitroanilid;    CD;    cluster of differentation;    EDTA;    ethylenediaminetetraacetic acid;    cpm;    counts per minute;    mab;    monoclonal antibody;    PBS;    phosphate-buffered saline;    PHA;    phytohemagglutinine;    PMA;    phorbol 12-acetate 13-myristate;    RT-PCR;    reverse transcription-polymerase chain reaction;    SDS;    sodium dodecyl sulfate;    SSC;    standard sodium citrate;   

DOI  :  10.1016/S0014-5793(97)00738-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Recently, it was shown that aminopeptidase N (E.C. 3.4.11.2, CD13) is up-regulated during mitogenic stimulation of peripheral T-cells. In this study, we demonstrate that the half-life of APN mRNA was considerably prolonged in these cells leading to a 2.7-fold increase of APN transcript level. The apparent half-life time of the APN transcript was investigated by the RNA synthesis inhibitor-chase method using actinomycin D. The steady-state APN mRNA levels was determined by a competitive RT-PCR. The half-lives estimated in resting T-cells, natural killer cells and permanently growing tumour cells varied between 3.5 and 6 h. Finally, nuclear run-on assays revealed that the APN gene expression of stimulated T-cells is controlled by increased promoter activity as well. These studies suggest a control of APN gene expression at the post-transcriptional level in addition to promoter-mediated regulation.

【 授权许可】

Unknown