[3H]Vasopressin binding to rat hippocampal synaptic plasma membrane

FEBS Letters

Barberis, C.¹ 

[1] Centre CNRS-INSERM de Pharmacologie Endocrinologie, BP 5055, 34033 Montpellier Cedex, France

关键词: Synaptic membrane;    Hippocampus;    Vasopressin binding;    Adenylate cyclase;   

DOI  :  10.1016/0014-5793(83)80795-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Characterization of specific vasopressin binding sites to rat hippocampal membranes has been assayed using tritiated lysine-vasopressin labelled on the tyrosyl residue. At 30°C specific [³H]vasopressin binding was saturable. The estimated equilibrium dissociation constant was 7.1 nM, the mean maximal binding capacity was 78 fmol/mg protein. Arginine-vasopressin has a high affinity (K _d = 2.8 nM) and dDAVP has a low affinity (K _d = 249 nM) for hippocampal synaptic membranes. (OH)AVP and Phe²Orn⁸VT are at least as active as AVP in inhibiting [³H]vasopressin binding. Adenylate cyclase was activated by VIP and inhibited by PIA, but not affected by lysine-vasopressin.

【 授权许可】

Unknown