Identification of barley and wheat cDNA clones related to the high‐M r polypeptides of wheat gluten

FEBS Letters

Kreis, M.¹  Shewry, P.R.¹  Forde, J.¹  Miflin, B.J.¹  Fry, R.P.¹  Forde, B.G.¹ 

[1] Department of Biochemistry, Rothamsted Experimental Station, Harpenden, Herts AL5 2JQ, England

关键词: D-Hordein;    Molecular cloning;    DNA-sequencing;   

DOI  :  10.1016/0014-5793(83)80788-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have identified 3 cDNA clones related to the high-M _r group of storage proteins in barley endosperm, the D-hordeins. A cDNA library has been constructed from wheat endosperm poly(A⁺)-RNA and screened using one of the D-hordein cDNA clones. Two wheat clones which cross-hybridised to the barley clone have been identified, by hybrid-release translation and nucleotide sequence analysis, as partial copies of mRNAs encoding the high-M _r gluten polypeptides of wheat.

【 授权许可】

Unknown