A rat brain protein kinase phosphorylating specifically neurofilaments

FEBS Letters

Pierre, M.¹  Toru-Delbauffe, D.¹ 

[1] Unité de Recherche sur la Glande Thyroide et la Régulation Hormonale, INSERM U.96, 78 rue du Général Leclerc, 94270 Bicetre, France

关键词: Neurofilament;    Rat brain;    Protein kinase;    Casein kinase;   

DOI  :  10.1016/0014-5793(83)80761-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Protein kinase activities associated with neurofilament (cAMP, cGMP, Ca²⁺-independent) were almost completely extracted by 0.8 M KCl. Two activities were separated by either sucrose gradient ultracentrifugation of phosphocellulose chromatography. One of them phosphorylates specifically neurofilament proteins and preferentially the triplet (200 kDA, 145 kDa and 68 kDa) but neither casein nor type II_A histone. The second activity was identified as casein kinase I and does not catalyze the phosphorylation of neurofilament protein.

【 授权许可】

Unknown