Autophosphorylation of type 2 casein kinase TS at both its α‐ and β‐subunits

FEBS Letters

Brunati, Anna Maria¹  Pinna, Lorenzo A.¹  Meggio, Flavio¹ 

[1] Istituto di Chimica Biologica dell'Università di Padova, Via Marzolo 3, 35100 Padova, Italy

关键词: Protein kinase;    Casein kinase;    Protein phosphorylation;    Autophosphorylation;    Polyarginyl effector;    (Polyamine);   

DOI  :  10.1016/0014-5793(83)80967-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Rat liver casein kinase TS (Ck-TS) having quarternary structure α₂β₂, autophosphorylates at its 25 kDa, β-subunits, incorporating up to 1.2 mol P/mol enzyme. According to their effects on the autophosphorylation pattern the effectors of Ck-TS activity can be grouped into 3 classes: (i) inhibitors, like heparin, which also prevent the autophosphorylation of the β-subunit; (ii) stimulators possessing several amino groups (like spermine) which increase the autophosphorylation at the β-subunit; (iii) stimulators possessing several guanido groups, like protamines and related peptides, which prevent the phosphorylation of the β-subunit, while promoting the autophosphorylation of the 38 kDa α-subunit. In the presence of such polyarginyl effectors the 130 kDa Ck-TS is converted into forms with higher sedimentation coefficient.

【 授权许可】

Unknown