The presence of NADPH‐glyceraldehyde 3‐phosphate oxidoreductase in macrophages

FEBS Letters

Gershon, David¹  Ravid, Katy¹  Lavie, Lena¹ 

[1] Department of Biology, Technion-Israel Institute of Technology, Haifa, Israel

关键词: Macrophage;    NADPH-triose phosphate oxidoreductase;    Hexose monophosphate shunt;    D-Glyceraldehyde 3-phosphate;    D-Glyceraldehyde;    Chemiluminescence;    RB;    respiratory burst;    HMS;    hexose monophosphate shunt;    D-GA3P;    D-glyceraldehyde-3-phosphate;    D-GA;    D-glyceraldehyde;    Cl;    chemiluminescence;   

DOI  :  10.1016/0014-5793(83)81058-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The existence of an NADPH-oxidoreductase which utilizes D-glyceraldehyde 3-phosphate as substrate has been demonstrated in mouse peritoneal macrophages. D-Glyceraldehyde could also serve as substrate, albeit with a 10-fold lower efficiency. No NADH oxidation could be demonstrated with either substrate. Addition of D-glyceraldehyde to cultured macrophages increased the rate of activity of the hexose monophosphate shunt to about 65% of the level observed i zymosan A-stimulated macrophages. The possible involvement of the oxidoreductase in this phenomenon and in the inhibitory effect of D-glyceraldehyde on the production of oxygen free radicals by zymosan-stimulated cells is discussed.

【 授权许可】

Unknown