Coupling of polyphosphoinositide breakdown with calcium efflux in formyl‐methionyl‐leucyl‐phenylalanine‐stimulated rabbit neutrophils

FEBS Letters

Nakashima, Shigeru¹  Yano, Koh¹  Nozawa, Yoshinori¹ 

[1] Department of Biochemistry, Gifu University School of Medicine, Tsukasamachi-40, Gifu 500, Japan

关键词: Polyphosphoinositide;    Ca2+ mobilization;    Formyl-methionyl-leucyl-phenylalanine;    Rabbit neutrophil;    TPI;    phosphatidylinositol 4;    5-biphosphate;    DPI;    phosphatidylinositol 4-phosphate;    PI;    phosphatidylinositol;    PA;    phosphatidic acid;    FMLP;    formyl-methionyl-leucyl-phenylalanine;    PBS;    phosphate-buffered saline;    HEPES;    N-2-hydroxymethylpiperazine-N'-2-ethanesulfonic acid;    EDTA;    ethyleneglycol bis(β-aminoethylether)N;    N'-tetraacetic acid;    EDTA;    ethylenediaminetetraacetic acid;    HPTLC;    high-performance thin-layer chromatography;   

DOI  :  10.1016/0014-5793(83)81028-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Exposure of rabbit neutrophils to formyl-methionyl-leucyl-phenylalanine (FMLP) induced the efflux of ⁴⁵Ca²⁺ from pre-labeled cells which was almost complete within 30 s. On the other hand, FMLP-induced ⁴⁵Ca²⁺ influx did not become apparent until 60 s after stimulation. When [³H]arachidonic acid-labeled neutrophils were stimulated with FMLP, the radioactivities in phosphatidylinositol 4,5-biphosphate (TPI) and phosphatidylinositol 4-phosphate (DPI) significantly decreased in parallel with the induction of ⁴⁵Ca²⁺ efflux. In contrast, degradation of polyphosphoinositides in [³H]glycerol-labeled neutrophils was not significant until 60 s. Taken together, these results indicate that the early degradation of polyphosphoinositides, especially of those rich in arachidonic acid is closely associated with the initial efflux of calcium in FMLP-stimulated rabbit neutrophils. The study of resynthesis of polyphosphoinositides by measuring ³²P_i incorporation into these lipids is also presented.

【 授权许可】

Unknown