A new site‐specific endodeoxyribonuclease from Citrobacter freundii

FEBS Letters

Janulaitis, A.¹  Stakenas, P.¹  Berlin, Yu.A.² 

[1] Institute of Applied Enzymology, 232028 Vilnius, Fermentu 8, Lithuanian SSR, USSR;Shemyakin Institute of Bioorganic Chemistry, USSR Academy of Sciences, Moscow 117988, USSR

关键词: Citrobacter freundii;    site-specific endonuclease;    molecular cloning;   

DOI  :  10.1016/0014-5793(83)81009-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cfr10 I, a site-specific endonuclease from Citrobacter freundii strain RFL 10, was isolated. It recognizes and cleaves the family of related sequences: 5'Pu^↓CCGGPy to generate DNA fragments with 5' tetranucleotide extensions. Cfr10 I may be useful in molecular cloning experiments, especially in conjunction with other enzymes which generate the same terminal extensions.

【 授权许可】

Unknown