Detection of inactive precursors of β‐glucanases in Saccharomyces cerevisiae

FEBS Letters

Hernandez, Luis M.¹  Olivero, Isabel¹  Larriba, German¹ 

[1] Departamento de Microbiologia, Facultad de Ciencias, Universidad de Extremadura, Badajoz, Spain

关键词: β-Glucanases;    Saccharomyces cerevisiae;    Secretory mutant Activation;    Endoplasmic reticulum;    Precursor;   

DOI  :  10.1016/0014-5793(83)81005-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Accumulation and secretion of β-glucanases have been studied in vivo by using a thermosensitive secretory mutant of Saccharomyces cerevisiae blocked at the endoplasmic reticulum level (sec 18-1). When incubated at the restrictive temperature no accumulation of active glucanases was observed. Following a shift to permissive conditions in the presence of cycloheximide a rise in the internal activity took place. The increase in total glucanase activity was partially due to the activation of an exo-glucanase that hydrolyzes PNPG. It is concluded that glucanases are synthesized in inactive precursor forms and are converted to the active forms in their secretory pathway.

【 授权许可】

Unknown