Ca2+, calmodulin‐dependent phosphorylation of glycogen synthase by a brain protein kinase

FEBS Letters

Tanaka, Etsuro¹  Miyamoto, Eishichi¹  Iwasa, Takafumi¹  Yamamoto, Hideyuki¹  Fukunaga, Kohji¹ 

[1] Department of Pharmacology, Kumamoto University Medical School, Kumamoto 860, Japan

关键词: Ca2+;    Calmodulin-dependent protein kinase;    Glycogen synthase;    Protein phosphorylation;    Brain;   

DOI  :  10.1016/0014-5793(83)80723-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A Ca²⁺, calmodulin-dependent protein kinase from brain with a M _r of 640 000 is capable of phosphorylating glycogen synthase from skeletal muscle. The reaction was inhibited by the addition of 1 mM EGTA and 50 μM trifluoperazine, but not by protein kinase inhibitor and heparin. The amount of phosphate incorporated into glycogen synthase was 1.4 mol/mol subunit. The phosphorylation sites of glycogen synthase were cyanogen bromide-treated peptides CB-1 and CB-2 and only the seryl residue was phosphorylated.

【 授权许可】

Unknown