FEBS Letters | |
Identification of a calmodulin‐dependent glycogen synthase kinase in rabbit skeletal muscle, distinct from phosphorylase kinase | |
Woodgett, James R.1  Tonks, Nicholas K.1  Cohen, Philip1  | |
[1] Department of Biochemistry, University of Dundee, Dundee DD1 4HN, Scotland | |
关键词: Calmodulin; Glycogen synthase; Protein phosphorylation; Cyclic AMP; | |
DOI : 10.1016/0014-5793(82)81231-3 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
A glycogen synthase kinase that is completely dependent on Ca2+ and calmodulin has been identified in mammalian skeletal muscle, and purified ∼3000-fold by chromatography on phosphocellulose and calmodulin—Sepharose. The presence of 50 mM NaCl in the homogenisation buffer was critical for extraction of the enzyme. The calmodulin-dependent glycogen synthase kinase (app. M r 850 000) is distinct from myosin light-chain kinase and phosphorylase kinase, but phosphorylates the same serine residue on glycogen synthase as phosphorylase kinase. The physiological role of the enzyme is discussed.
【 授权许可】
Unknown
【 预 览 】
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