Identification of a calmodulin‐dependent glycogen synthase kinase in rabbit skeletal muscle, distinct from phosphorylase kinase

FEBS Letters

Woodgett, James R.¹  Tonks, Nicholas K.¹  Cohen, Philip¹ 

[1] Department of Biochemistry, University of Dundee, Dundee DD1 4HN, Scotland

关键词: Calmodulin;    Glycogen synthase;    Protein phosphorylation;    Cyclic AMP;   

DOI  :  10.1016/0014-5793(82)81231-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A glycogen synthase kinase that is completely dependent on Ca²⁺ and calmodulin has been identified in mammalian skeletal muscle, and purified ∼3000-fold by chromatography on phosphocellulose and calmodulin—Sepharose. The presence of 50 mM NaCl in the homogenisation buffer was critical for extraction of the enzyme. The calmodulin-dependent glycogen synthase kinase (app. M _r 850 000) is distinct from myosin light-chain kinase and phosphorylase kinase, but phosphorylates the same serine residue on glycogen synthase as phosphorylase kinase. The physiological role of the enzyme is discussed.

【 授权许可】

Unknown