Interaction of the chloroplast ATP synthetase with the photoreactive nucleotide 3′‐O‐(4‐benzoyl)benzoyl adenosine 5'‐diphosphate

FEBS Letters

Tiefert, Marjorie A.¹  Bar-Zvi, Dudy¹  Shavit, Noun¹ 

[1] Department of Biology, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel

关键词: Photoaffinity labeling;    ATPase;    Analog;    Tightly-bound nucleotide;    Chloroplast;   

DOI  :  10.1016/0014-5793(83)80973-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The photoreactive nucleotide 3'-O-(4-benzoyl)benzoyl ADP (BzADP) is not a substrate for photophosphorylation but is a strong competitive inhibitor (K _i 2-25μM) with respect to ADP and ATP in photophosphorylation or ATP hydrolysis and P_i-ATP exchange reactions, respectively. The analog binds tightly to the membrane-bound CF₁, competes with the right binding of ADP, and prevents the inactivation of the enzyme by tight binding of ADP. Upon irradiation with long wavelength ultraviolet light, the tightly bound BzADP becomes covalently attached to both the α- and β-subunits of the enzyme.

【 授权许可】

Unknown