Effect of pertussis toxin on hormonal responsiveness of rat hepatocytes

FEBS Letters

Adolfo García-Sáinz, J.²  Corvera, Silvia¹  Pushpendran, Choliparambil K.² 

[1] Departamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México, Apartado Postal 70-600, DF 04510 México;Departamento de Bioenergética, Centro de Investigaciones en Fisiología Celular DF 04510 México

关键词: Pertussis toxin;    α1-Adrenoceptor;    β2-Adrenoceptor;    Glucagon;    Ureogenesis;    Adenylate cyclase regulation;   

DOI  :  10.1016/0014-5793(83)80966-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The ureogenic action of epinephrine in hepatocytes from normal adult rats is mediated through activation of α₁-adrenoceptors. β-Adrenoceptors in addition to α₁-adrenoceptors, became involved in mediating this effect in cells from animals treated with pertussis toxin. The accumulation of cyclic-AMP in response to epinephrine or isoproterenol was markedly increased in hepatocytes from pertussis-treated rats as compared to that observed in control cells. The accumulation of cyclic-AMP due to glucagon was also increased. It is suggested that pertussis toxin may release a constraint on adenylate cyclase activity by blocking the inhibitory coupling mechanism (N _i) or some other entity involved in the regulation of the activity of this enzyme.

【 授权许可】

Unknown