Effects of glucose, anoxia, and adriamycin on the chemiluminescence of Ehrlich Ascites cells

FEBS Letters

Cheng, B.¹  Chance, B.¹  Williams, M.¹ 

[1] Department of Biophysics, Beijing Medical College, Beijing, China and Johnson Foundation, D-501 Richards g/4, University of Pennsylvania, Philadelphia, PA 19104, USA

关键词: Chemiluminescence;    Antibody;    Ehrlich Ascites;    Adriamycin;    Anoxia;    Glucose;    ADM;    adriamycin;    UDP;    uronyl diphosphate;    CL;    chemiluminescence;    EAC;    Ehrlich Ascites cells;   

DOI  :  10.1016/0014-5793(83)80959-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Glucose and anoxia accelerate the photocount due to luminescence of Ehrlich Ascites cells. Adriamycin also has this effect if glucose is present. Comparison with a chemical standard combined with estimates of cellular and population transmittance yield a photon generation rate of at least 10.s⁻¹.cell⁻¹ in the presence of 10⁻² M glucose, and twice this with anoxic conditions or 10⁻⁵ M adriamycin. Effects of adriamycin on Ehrlich Ascites cell respiration may depend on the presence of glucose.

【 授权许可】

Unknown