The study of the state [P870+ B800−] in bacterial reaction centers by selective picosecond and low‐temperature spectroscopies

FEBS Letters

Klevanik, A.V.¹  Shuvalov, V.A.¹ 

[1] Institute of Photosynthesis, USSR Academy of Sciences, 142292 Poustchino, Moscow Region USSR

关键词: Selective picosecond spectroscopy;    Bacterial reaction center;    Electron transfer;    Bacteriochlorophyll;    Bacteriopheophytin;   

DOI  :  10.1016/0014-5793(83)80934-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The selective picosecond excitation of Rhodopseudomonas sphaeroides (R-26) reaction centers (RCs) at 870 nm induces the formation of the transient state within <1 ps followed by the conversion into the state P^F (P^± Bph^±− during 7 ± 2 ps at both 293 K and 110 K. The transient state including the intense bleaching at 800 nm has been shown not to be due: (a) to photon excitation at 870 nm; (b) the excitation of P⁺; (c) photoselection effects. The transient state is interpreted as the state ¹[P⁺B⁻] in agreement with earlier works. The primary formation of the state ¹P⁺B⁻] and the big effective singlet-triplet splitting in this state correspond to the spectral splitting of the P band at 900 nm in R-26 RCs and at 1000 nm in Rhodopseudomonas viridis RCs found at 4.2 K and attributed to the optical transition to both ¹P and ¹[P⁺B⁻] states.

【 授权许可】

Unknown