Specific DNA binding of the cyclic AMP receptor protein to a synthetic oligodeoxyribonucleotide

FEBS Letters

Martin, Stephen R.¹  Clore, G.Marius¹  Gronenborn, Angela M.¹ 

[1] Division of Biophysics and Molecular Pharmacology, National Institute for Medical Research, Mill Hill, London NW7 1AA, England

关键词: CRP;    Specific DNA binding;    Synthetic oligonucleotide;    Circular dichroism;   

DOI  :  10.1016/0014-5793(83)80425-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The interaction of the cAMP receptor protein (CRP) of Escherichia coli with a synthetic DNA undecamer (11mer) comprising a portion of the specific target site in the gal operon and containing 8 basepairs out of the 10 basepair concensus making up specific CRP sites, has been studied by circular dichroism spectroscopy. The binding constants for the interaction of CRP with the 11mer in the presence and absence of cAMP have been determined, and it is shown that CRP, both in the presence and absence of cAMP, induces a B—C transition in the conformation of the 11mer.

【 授权许可】

Unknown