Chemical crosslinking of histone H1° to histone neighbours in nuclei and chromatin

FEBS Letters

Nikolaev, Lev G.¹  Zlatanova, Jordanka J.²  Glotov, Boris O.¹  Severin, Evgenii S.¹  Dashkevich, Viktor K.¹ 

[1] Institute of Molecular Biology, USSR Academy of Sciences, Moscow 117984, USSR;Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia 1113, Bulgaria

关键词: Chromatin structure;    Histone H1° location;    Protein crosslinking;    PMSF;    phenylmethylsulphonyl fluoride;    TEA;    triethanolamine;    Tris;    tris-(hydroxymethyl)-aminomethane;    EGTA;    ethylene glycol bis(2-aminoethyl ether)-N;    N′-tetraacetic acid;   

DOI  :  10.1016/0014-5793(83)80594-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Crosslinking of histones in mouse liver nuclei and extended chromatin with a bifunctional reagent leads to the formation of H1H1° heterodimers as well as H1°H1° homodimers. H1° can be also crosslinked to the core histones. Thus, the location of histone H1° within the basic repeating chromatin structure seems to be analogous to that of H1 histone.

【 授权许可】

Unknown