A rapid stimulation of phosphatidylinositol metabolism in rabbit leukocytes by pseudomonal leukocidin

FEBS Letters

Kato, Iwao¹  Hirayama, Toshiya¹ 

[1] Department of Bacterial Infection, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo 108, Japan

关键词: Phosphoinositol metabolism;    Phosphatidic acid;    Leukocidin;    Pseudomonas aeruginosa;    Rabbit leukocyte;    Toxin action;   

DOI  :  10.1016/0014-5793(83)81113-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The time course experiments of ³²P_i-labelling and breakdown of phospholipids in rabbit leukocytes exposed to leukocidin from Pseudomonas aeruginosa suggested that the initial action of this toxin was to stimulate phosphatidic acid production, presumably by causing a rapid metabolic change of phosphatidylinositol (PI response) correlating with phosphatidylinositol-specific phospholipase C and 1,2-diacylglycerol kinase. It appears that a rapid formation of phosphatidic acid and degradation of polyphosphoinositides in leukocytes treated with the toxin might be related a Ca²⁺-movement from extra- and intracellular spaces, resuling in the activation of Ca²⁺-dependent enzymes involved in the leukocidic process.

【 授权许可】

Unknown