Carbohydrate structures of hen ovomucoid

FEBS Letters

Fournet, Bernard¹  Paz-Parente, José¹  Egge, Heinz²  Strecker, Gerard¹  Montreuil, Jean¹  Peter-Katalinić, Jasna² 

[1] Laboratoire de Chimie Biologique de l'Université des Sciences et Techniques de Lille I and Laboratoire Associé au CNRS no.217, 59655 Villeneuve D'Ascq Cédex, France;Institut für Physiologische Chemie der Universität Bonn, FRG

关键词: Ovomucoid;    Carbohydrate structure;    Fast atom bombardment;    Heterogeneity;    Mass spectrometry;    amu;    atomic mass units;    FAB;    fast atom bombardment;    EI;    electron impact;    MS;    mass spectrometry;    NMR;    nuclear magnetic resonance;    MIKES;    mass analysed ion kinetic energy spectrometry;   

DOI  :  10.1016/0014-5793(83)80528-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The apparently homogenous N-glycosidically-linked glycans 1, 7, 11 and 14 released by hydrazinolysis from hen ovomucoid were analysed by fast atom bombardment and electron-impact mass spectrometry after reduction and permethylation. The spectra support the primary structures established independently [FEBS Letters (1983) 152, 145–152] using methylation analysis, partial acid hydrolysis and 500 MHz ¹H NMR spectroscopy. In addition to the major constituents present in fractions 1, 7, 11 and 14, four minor components not detected by other methods could be characterized with the aid of the mass spectrometry data as: Man₂GlcNAcGlcNAc-ol, GlcNAc₄Man₃GlcNAc-ol, GlcNAc₆Man₃GlcNAc-ol and GalGlcNAc₆Man₃GlcNAc-ol. Our results show that the physical techniques used provide valuable data on the structure of complex glycans. In addition they can be employed to ascertain the homogeneity of the compounds examined as well as to detect trace amounts of homologs that might not be noticed by other methods.

【 授权许可】

Unknown