The TolC protein of Escherichia coli K12 is synthesised in a precursor form

FEBS Letters

Hackett, Jim¹  Misra, Rajeev¹  Reeves, Peter¹ 

[1] Department of Microbiology, University of Adelaide, Adelaide, SA 5001, Australia

关键词: TolC protein;    Signal sequence;    E. scherichia coli outer membrane;    colicin El;    DNA sequencing;    OmpF protein;   

DOI  :  10.1016/0014-5793(83)80518-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We examined the biosynthesis of the TolC protein of Escherichia coli K12 in a pulse—chase experiment, followed by immunoprecipitation with anti-TolC antibody and SDS—PAGE of the immunoprecipitate. This showed that TolC protein was originally synthesised in a precusor form (M _r 54 500) which could be chased into the mature form (M _r 52 000). DNA sequencing of a portion of the cloned tolC gene showed that the N-terminus of the mature rotein was preceded by a typical signal sequence of 22 residues (M _r 2542). The initiator Met was preceded by a Shine—Dalgarno sequence, with the correct spacing.

【 授权许可】

Unknown