Human liver RNA‐programmed in vitro synthesis of a polypeptide related to human apolipoprotein B

FEBS Letters

Olofsson, Sven-Olof¹  Norfeldt, Per-Ingvar¹  Bondjers, Göran¹  Lundholm, Kent¹  Boström, Kristina¹  Wiklund, Olov¹  Elias, Per¹  Fager, Gunnar¹ 

[1] The Arterial Biology Group, Department of Medical Biochemistry, Department of Internal Medicine I and Department of Surgery I, University of Göteborg, Box 33031, S-400 33 Göteborg, Sweden

关键词: Apolipoprotein B;    Human liver RNA;    In vitro translation;    Low density lipoprotein;    DU;    density units;    LDL;    low density lipoproteins (d=1.019-1.063);    LDL (d=1.030-1.055);    a narrow density cut of LDL;    Lp-B;    lipoprotein B;    apo;    apolipoprotein;    apo LDL (d=1.030-1.055);    the protein component of a narrow density cut of LDL;    SD;    standard deviation;    SDS-PAGE;    sodium dodecyl sulphate-polyacrylamide gel electrophoresis;    PMSF;    phenylmethylsulfonylfluoride;   

DOI  :  10.1016/0014-5793(83)80249-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In an in vitro synthesizing system programmed with RNA from human liver a polypeptide with an estimated M _r of 80000 (80 kDa)±1400 (mean±SD, n=5) was synthesized. This polypeptide could be precipitated with antiserum to a narrow density cut of LDL (d=1.030-1.055) or antiserum against the high-M _r form of apoB (apoB 100 [4]). The synthesized protein is immunologically related to a 75 kDa protein isolated from LDL. We suggest that the 80 kDa protein represents a primary translation product of apoB synthesized in human liver.

【 授权许可】

Unknown