Liquid crystalline microphases of DNA molecules complexed with compounds of platinum(II)

FEBS Letters

Akimenko, N.²  Yevdokimov, Yu.²  Kleinwächter, V.¹ 

[1] Institute of Biophysics, Czechoslovak Academy of Sciences, Kralovopolska 135, 61265 Brno 12, Czechoslovakia;Institute of Molecular Biology, USSR Academy of Sciences, Ul. Vavilova 32, GSP 117984, Moscow V-334, USSR

关键词: Liquid crystalline microphase of DNA;    Platinum(II) complex;    Secondary structure of DNA;   

DOI  :  10.1016/0014-5793(83)80248-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The formation of liquid crystalline microphases (0.3 M NaClO₄, and 120 and 170 mg PEG/ml) from low-M _r DNA (salmon sperm) complexed with cis and trans dichlorodiamine-platinum(II) was investigated. It was shown that the amplitude of the negative band in the CD spectrum, characteristic of a liquid crystalline microphase of DNA, decreased upon complexing with platinum compounds. It was estimated that the influence of cis Pt(II) on the optical properties of liquid crystalline microphase of DNA molecules strongly differed from the effect of trans Pt(II); the phenomenon did not depend on [PEG]. The reasons of the decrease of the negative band in the CD spectra of the DNA liquid crystalline microphases are discussed.

【 授权许可】

Unknown