A novel method for the immobilisation and culture of plant cells

FEBS Letters

Black, G.M.²  Mavituna, F.²  Lindsey, K.¹  Yeoman, M.M.¹ 

[1] Botany Department, University of Edinburgh, Edinburgh, Scotland;Chemical Engineering Department, UMIST, Manchester, England

关键词: Cell immobilisation;    Secondary metabolite production;    Cell differentiation;    Polyurethane foam;    Capsicum frutescens Mill.;    Daucus carota L;   

DOI  :  10.1016/0014-5793(83)80227-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A novel technique is described for the immobilisation of plant cells. The method is simple and does not require the use of potentially toxic gels for the entrapment process. Liquid-suspended cells of two species, Capsicum frutescens Mill. and Daucus carota L. were found to invade, and were strongly retained in, polyurethane foam particles over a 21 day period. The viability of te immobilised cells was high (70–80% were alive after 21 days). On agitation of the loaded foam particles in fresh liquid medium, at least 95% of the cells remained immobilised after 3 or 4 days.

【 授权许可】

Unknown