期刊论文详细信息
FEBS Letters | |
A novel method for the immobilisation and culture of plant cells | |
Black, G.M.2  Mavituna, F.2  Lindsey, K.1  Yeoman, M.M.1  | |
[1] Botany Department, University of Edinburgh, Edinburgh, Scotland;Chemical Engineering Department, UMIST, Manchester, England | |
关键词: Cell immobilisation; Secondary metabolite production; Cell differentiation; Polyurethane foam; Capsicum frutescens Mill.; Daucus carota L; | |
DOI : 10.1016/0014-5793(83)80227-0 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
A novel technique is described for the immobilisation of plant cells. The method is simple and does not require the use of potentially toxic gels for the entrapment process. Liquid-suspended cells of two species, Capsicum frutescens Mill. and Daucus carota L. were found to invade, and were strongly retained in, polyurethane foam particles over a 21 day period. The viability of te immobilised cells was high (70–80% were alive after 21 days). On agitation of the loaded foam particles in fresh liquid medium, at least 95% of the cells remained immobilised after 3 or 4 days.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO201912020284230ZK.pdf | 680KB | download |