Muscarinic stimulation of phospholipid turnover in dissociated avian salt gland cells

FEBS Letters

Hootman, Seth R.¹  Heacock, Anne M.²  Fisher, Stephen K.²  Agranoff, Bernard W.²  Ernst, Stephen A.¹ 

[1] Department of Anatomy and Cell Biology, University of Michigan, Ann Arbor, MI 48109, USA;Neuroscience Laboratory, University of Michigan, 1103 East Huron, Ann Arbor, MI 48109, USA

关键词: Avian salt gland;    Muscarinic receptor;    Inositol phospholipid;    Phosphatidate;    Protein phosphorylation;    Secretion;   

DOI  :  10.1016/0014-5793(83)80205-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Addition of carbamylcholine to ³²P-prelabeled dissociated avian salt gland cells resulted in increased turnover of phosphatidic acid, phosphatidylinositol, phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bisphosphate, which could be prevented by the inclusion of atropine. Carbamylcholine had no discernable effect on protein phosphorylation, measured either in the total preparation or in subcellular fractions. It is concluded that for the avian salt gland, no obligatory link is indicated between protein phosphorylation and either phospholipid turnover or salt secretion.

【 授权许可】

Unknown