Oxygen‐derived radicals from Trypanosoma cruzi‐stimulated human neutrophils

FEBS Letters

Cardoni, Rita L.⁴  Mason, Ronald P.⁵  Madeira, Eliana D.³  Docampo, Roberto¹  Moreno, Silvia N.J.¹  Casellas, Angela M.² 

[1] Centro de Investigaciones Bioenergéticas, Facultad de Medicina, Universidad de Buenos Aires, Argentina;Instituto de Investigaciones Médicas, Facultad de Medicina, Universidad de Buenos Aires, Argentina;Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil;Departamento de Inmunohematología, Academia Nacional de Medicina, Buenos Aires, Argentina;National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA

关键词: DMPO;    Neutrophil;    Hydrogen peroxide;    Free radical;    Superoxide;    Trypanosoma cruzi;   

DOI  :  10.1016/0014-5793(83)80201-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

This study provides biochemical and electron spin resonance spectroscopic evidence that contract of human polymorphonuclear leukocytes with antibody-coated Trypanosoma cruzi triggers the respiratory burst. Oxygen consumption, superoxide anion and hydrogen peroxide release were stimulated under conditions of polymorphonuclear leukocyte-mediated killing. This stimulation did not occur under non-killing conditions when antibody was omitted. A common mechanism of cytotoxicity of human polymorphonuclear leukocytes against different T. cruzi forms is suggested by the triggering of the respiratory burst by antibody-coated epimastigotes and trypomastigotes.

【 授权许可】

Unknown