Generation of OH radical during enzymatic reduction of 9,10‐anthraquinone‐2‐sulphonate Can semiquinone decompose hydrogen peroxide?

FEBS Letters

Sushkov, D.G.¹  Gritsan, N.P.¹  Weiner, L.M.¹ 

[1] Institute of Chemical Kinetics and Combustion, Novosibirsk, 630090, USSR

关键词: Quinone;    NADPH-cytochrome P-450 reductase;    Hydroxyl radical;    Reaction mechanism;    Hydrogen peroxide;    FP;    NADPH-cytochrome P-450 reductase;    AQS;    9;    10-anthraquinone-2-sulphonate (Na salt);    DMSO;    dimethyl sulphoxide;    DMPO;    5;    5-dimethylpyrroline N-oxide;    SOD;    superoxide dismutase;   

DOI  :  10.1016/0014-5793(87)81146-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Spin-trapping experiments have shown that 9, 10-anthraquinone-2-sulphonate (AQS) can be reduced enzymatically with NADPH-cytochrome P-450 reductase (FP) producing OH radicals. It has been revealed for the first time that the radical anion of AQS cannot decompose H₂O₂ as indicated by flash-photolysis data. From the experimental results obtained it follows that an enzyme system containing NADPH, AQS and FP produces both accumulation of H₂O₂ from molecular oxygen and reduction of Fe³⁺ to Fe²⁺ which decomposes H₂O₂ catalytically via a Fenton reaction.

【 授权许可】

Unknown