Possible involvement of NADPH‐cytochrome P450 reductase and cytochrome b 5 on β‐ketostearoyl‐CoA reduction in microsomal fatty acid chain elongation supported by NADPH

FEBS Letters

Ishibashi, Teruo¹  Okayasu, Takako¹  Nagao, Masaki¹  Imai, Yoh¹ 

[1] Department of Biochemistry, Hokkaido University School of Medicine, Sapporo 060, Japan

关键词: Immunochemical evidence;    Cytochrome b 5;    FpT;    Microsome;    Fatty acid;    Chain elongation;   

DOI  :  10.1016/0014-5793(83)80198-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The chain elongation products from [2-¹⁴C]malonyl-CoA and palmitoyl-CoA by rat liver microsomes initiated by NADPH were found mainly to be 18:1 and 18:0. When anti-NADPH-cytochrome P450 reductase or anti-cytochrome b ₅ immunoglobulin G were included in this system, not only was the overall chain elongation significantly suppressed, but also a new radioactive product, which was identified as 2-heptadecanone derived from an intermediate β-ketostearate, is accumulated depending upon the amounts of IgG added. These results suggest that NADPH-cytochrome P450 reductase and cytochrome b ₅ participate in the conversion from β-ketostearoyl-CoA to β-hydroxystearoyl-CoA, which is the first reductive step of the microsomal chain elongating system.

【 授权许可】

Unknown