Myosin light chain phosphorylation during contraction of turtle heart

FEBS Letters

Sayers, S.T.¹  Bárány, K.¹ 

[1] Department of Physiology and Biophysics, College of Medicine, University of Illinois at Chicago, Chicago, IL 60612, USA

关键词: Myosin light chain;    Light chain phosphorylation;    Cardiac muscle;    Contractility;   

DOI  :  10.1016/0014-5793(83)80172-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The phosphorylation of myosin P-light chain was determined during the contraction cycle of turtle heart beating 5–8 times/min at 5°C. The hearts were freeze-clamped either in systole or diastole, then homogenized and washed in strong acids in order to completely inhibit myosin light chain kinase and phosphatase and isolate the total P-light chain of the heart. The phospho and dephospho forms of P-light chain were separated by two-dimensional gel electrophoresis and were quantitated by densitometry. Alternatively, the hearts were perfused with ³²P and the incorporation of [³²P]phosphate into the P-light chain was determined. Both methods demonstrated that in hearts frozen in systole more P-light chain was phosphorylated than in hearts frozen in diastole.

【 授权许可】

Unknown