Reticulocyte lipoxygenase exhibits both n‐6 and n‐9 activities

FEBS Letters

Schewe, T.¹  Kühn, H.¹  Rapoport, S.M.¹  Wiesner, R.¹ 

[1] Humboldt University Berlin, Institute of Physiological and Biological Chemistry, Hessische Str. 3–4, DDR-104 Berlin, GDR

关键词: Lipoxygenase;    Positional specificity;    Reticulocytes;    15-Hydroperoxyeicosatetraenic acid;    12-Hydroperoxyeicosatetranoic acid;    Arachidonic acid;   

DOI  :  10.1016/0014-5793(83)80641-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Purified reticulocyte lipoxygenase converts arachidonic acid to both 15- and 12-hydroxyperoxyeicosatetraenoic acids. The proportion of the two reaction products does not change during the purification procedure as shown by HPLC analysis. By means of isoelectric focusing it was not possible to separate the n-6 and n-9 activities. Reticulocyte lipoxygenase was completely inactivated by both 5,8,11-eicosatriynoic and 5,8,11,14-eicosatetraynoic acids in contrast to soybean lipoxygenase-1 which inactivated only by 5,8,11,14-eicosatetraynoic acid. These results indicate that reticulocyte lipoxygenase exhibits both n-6 and n-9 activities. A contamination of the enzyme preparation with other lipoxygenases, e.g., the n-9 lipoxygenase from thrombocytes appears to be excluded.

【 授权许可】

Unknown