FEBS Letters | |
A jump in an Arrhenius plot can be the consequence of a phase transition | |
Travers, F.1  Barman, T.E.1  Biosca, J.A.1  | |
[1] INSERM U 128, B.P. 5051, 34033 Montpellier Cedex, France | |
关键词: Arrhenius plots; discontinuous; Phase transition; Myosin subfragment 1; ATP binding to; Conformational change; Enzyme kinetics; | |
DOI : 10.1016/0014-5793(83)80151-3 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The temperature dependence of the kinetics of the binding of ATP to myosin subfragment−1 was studied by an ATP chase technique in a rapid−flow—quench apparatus: A temperature range of 30°C to −15°C was obtained with ethylene glycol as antifreeze. The Arrhenius plot of k 2 is discontinuous with a jump at 12°C. Above the jump ΔH ≠ = 9.5 kcal/mol, below ΔH ≠ = 28.5 kcal/mol. Few such Arrhenius plots are recorded in the literature but they are predicted from theory. Thus, we explain our results as a phase change of the subfragment 1−ATP system at 12°C. This is in agreement with certain structural studies.
【 授权许可】
Unknown
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