Calorimetric characterization of the stable complex of myosin subfragment 1 with ADP and beryllium fluoride

FEBS Letters

Golitsina, Nina L.¹  Bobkov, Andrey A.²  Khvorov, Nikolai V.¹  Levitsky, Dmitrii I.² 

[1] A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow 119899, Russian Federation;A.N. Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow 117071, Russian Federation

关键词: Myosin subfragment 1;    Beryllium fluoride;    Scanning microcalorimetry;    Rabbit skeletal muscle;   

DOI  :  10.1016/0014-5793(93)80485-D

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The thermal unfolding of the myosin subfragment 1 (S1) in its stable complex with ADP and beryllium fluoride (S1 · ADP · BeF₃ ⁻) was studied by differential scanning calorimetry. It has been shown that the structure of the S1 molecule in the S1 · ADP · BeF₃ ⁻ complex is similar to that of S1 in its complex with ADP and orthovanadate (S1 · ADP · V_i but differs radically from that of nucleotide-free S1 and S1 in the S1 · ADP complex. It is concluded that the S1 · ADP · BeF₃ ⁻ complex can be considered, like the S1 · ADP · V_i complex, a stable structural analogue of the myosin head · ADP · P_i transition state of the myosin-catalyzed ATP hydrolysis.

【 授权许可】

Unknown