FEBS Letters | |
Calorimetric characterization of the stable complex of myosin subfragment 1 with ADP and beryllium fluoride | |
Golitsina, Nina L.1  Bobkov, Andrey A.2  Khvorov, Nikolai V.1  Levitsky, Dmitrii I.2  | |
[1] A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow 119899, Russian Federation;A.N. Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow 117071, Russian Federation | |
关键词: Myosin subfragment 1; Beryllium fluoride; Scanning microcalorimetry; Rabbit skeletal muscle; | |
DOI : 10.1016/0014-5793(93)80485-D | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The thermal unfolding of the myosin subfragment 1 (S1) in its stable complex with ADP and beryllium fluoride (S1 · ADP · BeF3 −) was studied by differential scanning calorimetry. It has been shown that the structure of the S1 molecule in the S1 · ADP · BeF3 − complex is similar to that of S1 in its complex with ADP and orthovanadate (S1 · ADP · Vi but differs radically from that of nucleotide-free S1 and S1 in the S1 · ADP complex. It is concluded that the S1 · ADP · BeF3 − complex can be considered, like the S1 · ADP · Vi complex, a stable structural analogue of the myosin head · ADP · Pi transition state of the myosin-catalyzed ATP hydrolysis.
【 授权许可】
Unknown
【 预 览 】
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RO201912020298545ZK.pdf | 300KB | download |