Protein‐synthesis inhibitory protein from seeds of Luffa cylindria roem

FEBS Letters

Kishida, Kazuo¹  Hara, Takeshi¹  Masuho, Yasuhiko¹ 

[1] Teijin Institute for Biomedical Research, Asahigaoka, Hino, Tokyo 191, Japan

关键词: Luffa cylindria roem;    Luffin;    Protein synthesis;    Inhibition of protein synthesis;    Rabbit reticulocyte lysate;    Cytotoxicity;    Buffer I;    20 mM Na phosphate buffer (pH 7.2) containing 0.2 M NaCl;    FBS;    fetal bovine serum;    2-ME;    2-mercaptoethanol;    PAP;    Phytolacca americana antiviral protein;    PBS;    10 mM phosphate buffer (pH 7.2) containing 0.14 M NaCl;    SDS—PAGE;    sodium dodecylsulfate—polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(83)80149-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A protein (designated as luffin) with an app. M _r of 26000, which inhibits protein synthesis in rabbit reticulocyte lysate, was purified to homogeneity from the seeds of Luffa cylindria roem by extraction with 20 mM Na phosphate buffer (pH 7.2) containing 0.2 M NaCl, ammonium sulfate fractionation, and chromatography on Sephacryl S-200 and CM-Sephadex C-25. Luffin exhibited 10-times as strong inhibitory activity against protein synthesis in rabbit reticulocyte lysate (IC ₅₀, 0.42 ng/ml) as that of ricin A-chain, but it showed only a weak cytotoxicity against murine leukemia L1210 cells, an activity of 1/10⁶ to 1/10⁵ that of ricin.

【 授权许可】

Unknown