Interaction of homo‐aza‐steroidal ester of [p‐[bis‐(2‐chloroethyl) amino]phenyl]acetic acid (ASE) with DNA of Ehrlich ascites tumor cells

FEBS Letters

Boutis, L.¹  Catsoulakos, P.²  Markov, G.G.²  Koliais, S.I.¹  Ivanov, I.G.²  Papageorgiou, A.¹ 

[1]Theagenion Cancer Institute, Thessaloniki and Laboratory of Pharmaceutical Chemistry, University of Patras, Patras, Greece

[2]Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia, Bulgaria

关键词: Homo-aza-steroidal ester;    Nitrogen mustard;    Ehrlich ascites tumor cell;    Hydroxyapatite;    S1-nuclease;    Reduction with sodium borohydride;   

DOI  :  10.1016/0014-5793(83)80146-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A cytostatic, homo-aza-steroidal ester of [p-[bis-(2-chloroethyl) amino]phenyl]acetic acid (ASE) was reduced with NaB³H₄ and [³H]ASE-treated DNA prepared in vitro. We found that: (1) ASE reacts preferentially with purines; (2) ASE decreases the thermal stability of the double helix upon binding to DNA; (3) [³H]ASE binding sites are clustered along the DNA molecules; (4) ASE binding sites probably represent oligo- or polypurine sequences.

【 授权许可】

Unknown