In vitro formation of different tubulin polymers from purified tubulin of Ehrlich ascites tumor cells

FEBS Letters

Flemming, M.¹  Doenges, K.H.¹  Schroeter, D.¹  Zimmermann, H.-P.¹ 

[1]Institute of Cell and Tumor Biology, German Cancer Research Center, PO Box 101949, D-6900 Heidelberg, FRG

关键词: Microtubules;    10-nm Fiber;    Ehrlich ascites tumor cell;    Second β-tubulin;    Tubulin-heterogeneity;   

DOI  :  10.1016/0014-5793(83)80088-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Preparations of cycled tubulin from Ehrlich ascites tumor cells contain several acessory proteins; once or twice cycled microtubule preparations are usually composed of fibers 10 nm in diameter, but lack vimentin. Highly purified tubulin consists of α- and β-tubulin and a minor component which was identified by peptide mapping as a second β-chain. This pure tubulin is able to form in vitro at low concentrations (1 mg protein/ml) fibers of about 10 nm width, and at higher concentrations (3.5 mg protein/ml) normal microtubules.

【 授权许可】

Unknown