FEBS Letters | |
Calcium inhibition of a heat‐stable cyclic nucleotide phosphodiesterase from Neurospora crassa | |
Shaw, Nicholas M.1  Harding, Roy W.1  | |
[1] Radiation Biology Laboratory, Smithsonian Institution, 12441 Parklawn Drive, Rockville, MD 20852-1773, USA | |
关键词: Neurospora crassa; Cyclic nucleotide phosphodiesterase; Ca2+ inhibition; Heat stability; PMSF; phenylmethylsulfonyl fluoride; EGTA; ethyleneglycol-bis-(β-aminoethyl ether) N; N′-tetraacetic acid; TLC; thin-layer chromatography; | |
DOI : 10.1016/0014-5793(83)80399-8 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Neurospora crassa had a heat-stable (up to 95°C), soluble cyclic nucleotide phosphodiesterase (PDE). Both unheated and heat-stable PDE activities were inhibited by micromolar concentrations of Ca2+. This inhibition was reversed by EGTA or EDTA in molar excess of the Ca2+ concentration. Calmodulin was not involved in the Ca2+ inhibition, nor was Ca2+ inhibition of the heat-stable PDE due to cleavage inactivation of the enzyme by a Ca2+-stimulated protease. In addition to Ca2+, several other cations inhibited the activity of the heat-stable enzyme. Cyclic AMP and cGMP, but not 2′3′ cAMP were substrates for both unheated and heat-stable PDEs. This is the first report of a PDE which is inhibited by micromolar concentrations of Ca2+.
【 授权许可】
Unknown
【 预 览 】
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