Organization of ribosomal DNA in yellow lupine (Lupinus luteus) and sequence of the 5.8 S RNA gene

FEBS Letters

Rafalski, Jan Antoni²  Söll, Dieter¹  Wiewiórowski, Maciej² 

[1] Department of Molecular Biophysics and Biochemistry, Yale University, PO Box 6666, New Haven CT 06511, USA;Institute of Bioorganic Chemistry, Polish Academy of Sciences, PL-61704 Poznan, Noskowskiego 12, Poland

关键词: Lupinus luteus RNA gene;    5.8 S RNA;    Ribosomal repeat;    Ribosomal DNA;    Plant gene;    DNA sequence;    kb;    kilobases;    rRNA;    ribosomal RNA;    1 × SSC buffer;    0.15 M NaCl;    0.015 M sodium citrate (pH 7.0);   

DOI  :  10.1016/0014-5793(83)80388-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The large ribosomal repeat of the Lupinus luteus genome is 8.8 kb in length as revealed by Southern blotting and hybridization to 5.8 S rRNA and cloned rDNA fragments of L. luteus and Schizosaccharomyces pombe. It codes for 18 S,5.8 S and 28 S rRNAs. A cloned 3.7 kb EcoR1 subfragment of the rDNA repeat, coding for 5.8 S rRNA and a large portion of the 28 S rRNA, has been sequenced in the region of 5.8 rRNA gene. The predicted rRNA sequence is homologous to broad bean 5.8 S rRNA and can be arranged in the generalized model of 5.8 S RNA secondary structure.

【 授权许可】

Unknown