期刊论文详细信息
FEBS Letters | |
Organization of ribosomal DNA in yellow lupine (Lupinus luteus) and sequence of the 5.8 S RNA gene | |
Rafalski, Jan Antoni2  Söll, Dieter1  Wiewiórowski, Maciej2  | |
[1] Department of Molecular Biophysics and Biochemistry, Yale University, PO Box 6666, New Haven CT 06511, USA;Institute of Bioorganic Chemistry, Polish Academy of Sciences, PL-61704 Poznan, Noskowskiego 12, Poland | |
关键词: Lupinus luteus RNA gene; 5.8 S RNA; Ribosomal repeat; Ribosomal DNA; Plant gene; DNA sequence; kb; kilobases; rRNA; ribosomal RNA; 1 × SSC buffer; 0.15 M NaCl; 0.015 M sodium citrate (pH 7.0); | |
DOI : 10.1016/0014-5793(83)80388-3 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The large ribosomal repeat of the Lupinus luteus genome is 8.8 kb in length as revealed by Southern blotting and hybridization to 5.8 S rRNA and cloned rDNA fragments of L. luteus and Schizosaccharomyces pombe. It codes for 18 S,5.8 S and 28 S rRNAs. A cloned 3.7 kb EcoR1 subfragment of the rDNA repeat, coding for 5.8 S rRNA and a large portion of the 28 S rRNA, has been sequenced in the region of 5.8 rRNA gene. The predicted rRNA sequence is homologous to broad bean 5.8 S rRNA and can be arranged in the generalized model of 5.8 S RNA secondary structure.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201912020283987ZK.pdf | 722KB | download |