Mechanism of caffeine‐induced inhibition of DNA synthesis in Escherichia coli

FEBS Letters

Sjåstad, Knut¹  Sandlie, Inger¹  Kleppe, Kjell¹  Lossius, Ivar¹ 

[1] Department of Biochemistry, University of Bergen, Bergen, Norway

关键词: Caffeine;    DNA synthesis;    Toluene-treated E. coli;    DNA enzymes;    dNMP;    any of the 4 deoxynucleoside monophosphates;   

DOI  :  10.1016/0014-5793(83)80077-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Caffeine inhibited DNA synthesis in toluene-treated Escherichia coli K 12 strains to the same extent as in intact cells using the incorporation of [³H]thymidine as a measure of DNA synthesis. The inhibition was found to be competitive with ATP, and it was not influenced by the concentrations of deoxynucleoside triphosphates to any extent. When caffeine was added together with other DNA synthesis inhibitors such as novobiocin, nalidixic acid or actinomycin D, the inhibition in all cases was non-additive. It is suggested that caffeine inhibits one of the ATP-requiring enzymes in the DNA replication machinery, possibly DNA polymerase III or one of the DNA helicases.

【 授权许可】

Unknown