N‐terminal amino acid sequences of precursor and mature forms of α‐1‐antitrypsin

FEBS Letters

Plummer, Thomas H.¹  Witt, Irene²  Heinrich, Peter C.¹  Schmelzer, Elmon³  Kaiser, Clemens²  Tran-Thi, Thuy-Anh³  Gross, Volker³ 

[1] Center for Laboratories and Research, New York State Department of Health, Albany, New York, USA;Kinderklinik der Universität, Mathildenstr. 1, D-7800 Freiburg, West Germany;Biochemisches Institut, Hermann-Herder-Str. 7, D-7800 Freiburg, West Germany

关键词: α1-Antitrypsin;    Edman degradation;    In vitro translation;    Prepeptide;    Hepatocyte primary culture;    Deglycosylation;   

DOI  :  10.1016/0014-5793(83)80069-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

α-1-Antitrypsin is found in hepatocytes as a high-mannose glycoprotein (M _r 49000), extracellularly as a complex-type glycoprotein (M _r 54000). Deglycosylation of both forms with peptide: N-glycosidase led to proteins of identical app. M _r (41000). The sequence of 26 N-terminal amino acids of rat α₁-antitrypsin was determined. A high content of polar amino acids was found. The partially characterized presequence of in vitro synthesized α₁-antitrypsin showed a cluster of hydrophobic amino acids. A pre-peptide of 24 amino acids is proposed. There is no evidence for the existence of a propeptide.

【 授权许可】

Unknown