Analytical isoelectric focusing of rat intestinal brush‐border enzymes: postnatal changes and effect of neuraminidase in vitro

FEBS Letters

Zákostelecka, Marie²  Lojda, Zdeněk³  Kolínska, Jiřina²  Kraml, Jiří¹  Kadlecová, Libuše¹ 

[1] 1st Department of Medical Chemistry, Kateřinská 32, 121 08 Prague 2, Czechoslovakia;Institute of Microbiology, Czechoslovak Academy of Sciences, Videňská 1083, 142 20 Prague 4, Czechoslovakia;Laboratory of Histochemistry, Charles University, Faculty of Medicine, Kateřinská 32, 121 08 Prague 2, Czechoslovakia

关键词: Brush-border enzyme;    Isoelectric focusing;    pI changes;    Sialic acid;    Postnatal development;    Rat small intestine;    Nap;    naphthyl;    2-NNap;    2-naphthyl-amide;    2-NNapOMe;    4-methoxy-2-naphthylamide;    Ind;    4-Cl-5-Br-3-indolyl;    CBZ-;    carbobenzoxy-;    FBB;    fast blue B salt;    HPR;    hexazonium-p-rosaniline;    HF;    hexazotized fuchsin;    IEF;    isoelectric focusing;   

DOI  :  10.1016/0014-5793(83)80067-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Increase in p1 values was found between 11 and 30 days of postnatal life in papain-solubilized brush-border enzymes of rat small intestine by means of thin-layer analytical isoelectric focusing in agarose gel. Treatment with neuraminidase converted the acidic forms of enzymes from 11- and 20-day-old rats into the zymogram patterns identical with the adult, more basic forms. The zymograms of the respective enzymes are the same in 30-, 60- and 74-day-old animals and do not change on treatment with neuraminidase.

【 授权许可】

Unknown