Isolation and characterization of the Co‐methyl and Co‐aquo derivative of 5‐hydroxybenzimidazolylcobamide (factor III) from Methanosarcina barkeri grown on methanol

FEBS Letters

Höllriegl, Ver²  Renz, Paul²  Scherer, Paul¹ 

[1] Institut für Allgemeine Botanik, Abteilung Mikrobiologie, Universität Hamburg, Ohnhorststraβe 18, D-2000 Hamburg 52, FRG;Institut für Biologische Chemie und Ernährungswissenschaft, Universität Hohenheim, Garbenstraβe 30, D-7000 Stuttgart 70, FRG

关键词: Co-methyl-5-hydroxybenzimidazolylcobamide;    Vitamin B12-factor III;    Methanosarcina barkeri;    Methane biosynthesis Methanol;    Archaebacterium;    5-hydroxybenzimidazolylcobamide;    factor III;   

DOI  :  10.1016/0014-5793(83)80363-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The Co-methyl and the Co-aquo derivative of 5-hydroxybenzimidazolylcobamide (factor III) were isolated as the major natural corrinoids from Methanosarcina barkeri ‘Fusaro’. They were characterized by their UV/VIS-spectra, their chromatographic behaviour, and their electrophoretic properties. The portion of Co-methyl-factor III was found to be 70–80% whereas Co-aquo-factor III contributed 20–30% to the total amount of corrinoids.

【 授权许可】

Unknown