A DNA fragment homologous to F1‐ATPase β subunit was amplified from genomic DNA of Methanosarcina barkeri Indication of an archaebacterial F‐type ATPase

FEBS Letters

Sato, Masa H.¹  Date, Takayasu²  Denda, Kimitoshi¹  Yoshida, Masasuke¹  Sumi, Masato¹ 

[1] Research Laboratory of Resources Utilization, Tokyo Institute of Technology, Nagatsuta, Yokohama, Japan;Department of Biochemistry, Kanazawa Medical University, Uchinada, Ishikawa, Japan

关键词: Archaebacteria;    Methanosarcina barkeri;    ATPase;    V-type ATPase;    F-type ATPase in archaebacteria;   

DOI  :  10.1016/0014-5793(92)81472-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A 490 bp DNA fragment was amplified from Methanosarcina barkeri genomic DNA by the polymerase chain reaction (PCR) using oligonucleotide primers designed based on conserved amino acid sequences of the F₁-ATPase β subunits. The amino acid sequence deduccd from the DNA sequence of this fragment was highly homologous to a portion of the F₁-ATPase β subunit. This indicates that this archaebacterium has a gene of F-type ATPase in addition to a gene of V-type ATPase.

【 授权许可】

Unknown