Identification of two discrete ribonucleoprotein particles within the monomer population of rat liver nuclear RNPs

FEBS Letters

Guialis, Apostolia¹  Arvanitopoulou, Anastasia¹  Patrinou-Georgoula, Meropi¹  Sekeris, Constant E.¹ 

[1] Biological Research Center, National Hellenic Research Foundation, 48 Vassileos Konstantinou Avenue Athens 501, Greece

关键词: Nuclear RNP;    HnRNP;    snRNP;    snRNA;    Agarose gel electrophoresis;    Rat liver;    snRNA;    small nuclear RNA;    RNP;    ribonucleoprotein;    DEP;    diethylpyrocarbonate;    SDS;    sodium dodecyl sulphate;   

DOI  :  10.1016/0014-5793(83)80357-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

30–50 S RNP particles (monoparticles) isolated from rate liver nuclei were submitted to electrophoresis in native 0.5% agarose gels. Two RNP fractions were thus separated, a minor one remaining closer to the top of the gel (MI) and a more abundant one migrating further into the gel (MII). SDS-polyacrylamide gel electrophoresis revealed that MII contains the major monoparticle (M _r 30 000–40 000 or ‘core’) polypeptides and higher molecular weight proteins, whereas MI contains several minor proteins of M _r >40 000. Some proteins are common to both particle classes. Urea-acrylamide gel electrophoresis revealed that HnRNA is mainly present in MII, whereas snRNA is confined to the MI particle class.

【 授权许可】

Unknown