Characterization of acid and alkaline phosphatase activity in preparations of tubulin and microtubule‐associated proteins

FEBS Letters

Prus, Karen¹  Wallin, Margareta¹ 

[1] Department of Zoophysiology, University of Göteborg, Box 250 59, S-400 31 Göteborg, Sweden

关键词: Tubulin;    Microtubule-associated protein;    Acid phosphatase;    Alkaline phosphatase;   

DOI  :  10.1016/0014-5793(83)80341-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Acid and alkaline phosphatase activity, determined by the hydrolysis of p-nitrophenyl phosphate, was found in preparation of microtubules purified from bovine brain by temperature-dependent assembly—disassembly and ion-exchange chromatography. Phosphocellulose-purified tubulin contained an associated acid phosphatase activity, stimulated by Mg²⁺ and Zn²⁺. Alkaline phosphatase activity with a pH optimum of 10.4 was measured in a fraction of microtubule-associated proteins (MAPs). Kinetics and the effects of sodium fluoride, sodium tartrate, sulfhydryl-blocking agents, EDTA and Zn²⁺ are reported.

【 授权许可】

Unknown