Purification and molecular properties of 3 polypeptides released from a highly active O2‐evolving photosystem‐II preparation by Tris‐treatment

FEBS Letters

Mitsuo, Nishimura¹  Shimada, Shoko¹  Yamamoto, Yasusi¹ 

[1] Department of Biology, Faculty of Science, Kyushu University 33, Fukuoka 812, Japan

关键词: O2 evolution;    Membrane protein;    Photosystem II;    Chloroplast Photosynthesis;    PS;    photosystem;    Tris;    tris(hydroxymethyl)aminomethane;    Mes;    2-(N-morpholino)-ethane- sulphonic acid;    SDS;    sodium dodecyl sulfate;    PAGE;    polyacrylamide gel electrohporesis;    A 280;    absorbance at 280 nm;    pI;    isoelectric point;    TMBZ;    3;    3′;    5;    5′-tetra-methylbenzidine;    CF1;    chloroplast coupling factor 1;    atrazine;    2-chloro-4-ethylamino-6-isopropylamino-s-triazine;   

DOI  :  10.1016/0014-5793(83)80340-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Tris-treatment of a highly active O₂-evolving photosystem-II preparation induced release of 3 polypeptides (M _r 33 000, 24 000 and 18 000), concomitant with inhibition of O₂ evolution [FEBS Lett. (1981)_133. 265-268]. The 3 polypeptides were purified with the use of electrofocusing. Isoelectric points of the proteins were 5.1, 6.5 and 9.2 in order of decreasing M _r value. Only a trace amount of histidine, cystein and methionine were detected in these proteins. Based on the amino acid compositions, polarity indexes of the proteins were calculated to be 47–49%, suggesting the 3 proteins to be hydrophilic.

【 授权许可】

Unknown